Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Kidney.| Product Name | KEAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.1) Padmanabhan, B., (2006) Mol. Cell 21 (5), 689-700. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KEAP1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| KEAP1 antibody can be used for detection of KEAP1 by ELISA at 1:312500. KEAP1 antibody can be used for detection of KEAP1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KEAP1 |
|---|---|
| Alternative Names | KEAP1 INrf2 KLHL19 |
| Molecular Weight(MW) | 70 kDa, 70 kDa |
Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Kidney.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | KEAP1 antibody can be used for detection of KEAP1 by ELISA at 1:312500. KEAP1 antibody can be used for detection of KEAP1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store KEAP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM282758