Application
Immunofluorescence of KEAP1 in human lung tissue with KEAP1 antibody at 20 μg/mL.Application
Western blot analysis of KEAP1 in human lung tissue lysate with KEAP1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | KEAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KEAP1 Antibody: KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κB activity by targeting IKKβ degradation. Mutation of the KEAP1 gene is found in lung cancer.1) Zhang DD, Lo SC, Cross JV, et al. Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex. Mol. Cell. Biol. 2004;24:10941-53. |
| Immunogen | KEAP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1. The immunogen is located within the last 50 amino acids of KEAP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| KEAP1 antibody can be used for detection of KEAP1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KEAP1 |
|---|---|
| Alternative Names | KEAP1 Antibody: INrf2 KLHL19 INRF2 KIAA0132 Kelch-like ECH-associated protein 1 Cytosolic inhibitor of Nrf2 INrf2 |
| Molecular Weight(MW) | 69 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | At least two isoforms of KEAP1 are known to exist. |
Application
Immunofluorescence of KEAP1 in human lung tissue with KEAP1 antibody at 20 μg/mL.
Application
Western blot analysis of KEAP1 in human lung tissue lysate with KEAP1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | KEAP1 antibody can be used for detection of KEAP1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | KEAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KEAP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM282757