Application
HEK293T cells transfected with either RC202424 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KHK antibody, and then analyzed by flow cytometry.Application
Anti-KHK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with KHKApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK.| Product Name | KHK antibody [1F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1F1] to KHKThis gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Full length human recombinant protein of human KHK(NP_000212) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1F1 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | KHK |
|---|---|
| Tissue Specificity | is specific for human KHK(NP_000212). |
Application
HEK293T cells transfected with either RC202424 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KHK antibody, and then analyzed by flow cytometry.
Application
Anti-KHK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with KHK
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK.| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
