Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-MKI67 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKI67 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKI67.| Product Name | Ki67 antibody [3D11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3D11] to Ki67Thought to be required for maintaining cell proliferation. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 1-250 of human MKI67. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3D11 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PWB |
| Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KIA, Ki-67, Ki67, MKI67, Ki 67 |
|---|---|
| Target | Ki67 |
| Tissue Specificity | is specific for amino acids 1-250 of human MKI67. |
| Cellular Localization | Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes. |
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-MKI67 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-MKI67mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKI67 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKI67.| Application Notes | Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
