Application
Immunohistochemical analysis of paraffin-embedded lung cancer (left) and rectal cancer (right) using KI67 mouse mAb with DAB staining.Application
Western blot analysis using KI67 mouse mAb against Hela (1), MCF-7 (2) and Raji (3) cell lysate| Product Name | Ki67 antibody [8D5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [8D5] to Ki67This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009] |
| Immunogen | CEDLAGFKELFQTPG |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8D5 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. Not yet tested in other applications. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MKI67 , KIA |
|---|---|
| Target | Ki67 |
| Molecular Weight(MW) | 358 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded lung cancer (left) and rectal cancer (right) using KI67 mouse mAb with DAB staining.
Application
Western blot analysis using KI67 mouse mAb against Hela (1), MCF-7 (2) and Raji (3) cell lysate| Application Notes | Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. Not yet tested in other applications. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
