| Product Name | Kinesin antibody [IBII] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [IBII] to KinesinKinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function. |
| Immunogen | Bovine brain kinesin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | IBII |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/500. GTX11262 recognizes kinesin in denatured and reduced tissue or cell preparations of the following species: rat (brain), bovine (microtubules or kinesin enriched brain), sea urchin eggs (microtubules). In kinesin-enriched rat brain preparation, 2 bands are detected at approximately 130-140 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | RatSea UrchinBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC133806 |
|---|---|
| Target | Kinesin |
| Cellular Localization | Microtubules |
| Application Notes | ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/500. GTX11262 recognizes kinesin in denatured and reduced tissue or cell preparations of the following species: rat (brain), bovine (microtubules or kinesin enriched brain), sea urchin eggs (microtubules). In kinesin-enriched rat brain preparation, 2 bands are detected at approximately 130-140 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Raw Ascites |
