| Product Name | KSR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to KSRKSR was initially considered to be a protein kinase acting in the Ras pathway, but has more recently been demonstrated to act as a scaffold protein, facilitating complex formation between RAF and MEK and ERK. |
| Immunogen | Fusion protein, corresponding to amino acids 118-248 of Rat KSR. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Endogenous levels of KSR may be too low to be detected by WB. Immunoprecipitation prior to WB is recommended for detection of endogenous KSR. Use at a concentration of 4 μg/ml after using 10ug to immunoprecipitate KSR from 1mg of 3T3/A31 RIPA lysate. Predicted molecular weight: 120 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | DYRK3 8444 DYRK5 KSR RED O43781 REDK 603497 |
|---|---|
| Target | KSR |
| Positive Control | 3T3/A31 RIPA lysate |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Endogenous levels of KSR may be too low to be detected by WB. Immunoprecipitation prior to WB is recommended for detection of endogenous KSR. Use at a concentration of 4 μg/ml after using 10ug to immunoprecipitate KSR from 1mg of 3T3/A31 RIPA lysate. Predicted molecular weight: 120 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 30% Glycerol, 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4 |
