Application
Detection of human Ku70 protein by Western Blot using GeneTex Ku70 polyclonal antiserum in HeLa whole cell extract (lane 1) and Raji whole cell lysate (lane 2).| Product Name | Ku70 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse polyclonal antiserum to human Ku70Originally identified as an autoantigen, the Ku protein are nuclear proteins found in eukaryotes. They exist as a heterodimer consisiting of Ku 70 and Ku 80, migrating at approximately 70 and 80 kDa respectively. They bind to DNA ends at double strand breaks. The Ku proteins interact with the DNA-dependent protein kinase (DNA-PK) and participate in the targeting of DNA-PK to DNA. The Ku proteins may participate in numerous cellular processes, including DNA repair and replication, cell signalling, cell proliferation, transcriptional activation and apoptosis. |
| Immunogen | Recombinant antigen containing the amino terminal portion of human Ku70 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Ku 70 DNA PKcs ligase |
|---|---|
| Target | Ku70 |
| Cellular Localization | Nuclear |
Application
Detection of human Ku70 protein by Western Blot using GeneTex Ku70 polyclonal antiserum in HeLa whole cell extract (lane 1) and Raji whole cell lysate (lane 2).| Positive Control | HeLa, Raji |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
