Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ku70 antibody (green) at 1:2000 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2000.Application
Detection of human Ku70 protein in HeLa whole cell lysate using GeneTex Ku70 529 monoclonal antibodyApplication
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE diluted at 1:5000Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE diluted at 1:1000| Product Name | Ku70 antibody [529] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody to Human Ku70Originally identified as an autoantigen, the Ku protein are nuclear proteins found in eukaryotes. They exist as a heterodimer consisiting of Ku 70 and Ku 80, migrating at approximately 70 and 80 kDa respectively. They bind to DNA ends at double strand breaks. The Ku proteins interact with the DNA-dependent protein kinase (DNA-PK) and participate in the targeting of DNA-PK to DNA. The Ku proteins may participate in numerous cellular processes, including DNA repair and replication, cell signalling, cell proliferation, transcriptional activation and apoptosis. |
| Immunogen | Recombinant antigen containing the amino terminal portion of human Ku70 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 529 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:500-1:5000. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CTC75, CTCBF, G22P1, KU70, ML8, TLAA |
|---|---|
| Target | Ku70 |
| Molecular Weight(MW) | 70 kDa (note) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ku70 antibody (green) at 1:2000 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2000.
Application
Detection of human Ku70 protein in HeLa whole cell lysate using GeneTex Ku70 529 monoclonal antibody
Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE diluted at 1:5000
Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE diluted at 1:1000| Positive Control | Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:5000. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
