Application
Immunocytochemistry/Immunofluorescence: Ku80 Antibody (5D5.8) - The Ku80 antibody was tested in HeLa cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).Application
Western Blot: Ku80 Antibody (5D5.8) - WB analysis of Ku80 in MCF7 cell lysate using .| Product Name | Ku80 antibody [5D5.8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [5D5.8] to Ku80Eukaryotic cells contain an abundant nuclear protein, Ku, which was initially discovered as an autoantigen. Ku is a heterodimer of 70 and 80 kDa subunits which binds to DNA ends and other alterations in double-stranded DNA including hairpins, nicks and gaps. Ku plays a role in DNA repair as an essential subunit of DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors (Sp1, Oct-1 and p53). Ku targets DNA-PK to DNA. The Ku/DNA-PK complex might provide a structural framework for holding broken chromosomes together during repair. Ku plays a role in many diverse processes including cell signaling, proliferation, DNA repair, replication, transcriptional activation and apoptosis. |
| Immunogen | HeLa cell extracts |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5D5.8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:500. Predicted molecular weight 85 kDa. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | ATP dependant helicase II 80kDa subunit antibody Anti-ATP dependant helicase II 80kDa subunit Anti-CTC box binding factor 85kDa subunit Anti-CTC85 Anti-CTCBF Anti-DNA repair protein XRCC5 Anti-Ku autoantigen Anti-Ku80 Anti-Ku85 Anti-Lupus Ku autoantigen p |
|---|---|
| Target | Ku80 |
| Tissue Specificity | This antibody is specific for Ku 80 subunit. |
Application
Immunocytochemistry/Immunofluorescence: Ku80 Antibody (5D5.8) - The Ku80 antibody was tested in HeLa cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).
Application
Western Blot: Ku80 Antibody (5D5.8) - WB analysis of Ku80 in MCF7 cell lysate using .| Positive Control | HeLa whole cell extract |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500. Predicted molecular weight 85 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.05% sodium azide |
