Application
FACS analysis of K562 cells using Kv11.1 antibody (black) or an isotype control (green).Application
ICC/IF analysis of Kv11.1 transfected HEK293 cells using Kv11.1 antibody at a dilution of 1:25 (red) in the absence (left) or presence (right) of blocking peptide. Nuclei were stained with Hoechst 33342 (blue).Application
IP analysis was performed with Kv11.1 expressing HEK293 lysates and Kv11.1 antibodies (lane 2), (lane 3), (lane 4) or pre-immune serum (lane 5). Control lysate (lane 1) and the precipitates were detected by . Red arrow indicates Kv11.1 while the black arrow shows the IgG heavy chain.Application
IHC analysis of rat heart using Kv11.1 antibody. (A) Transversal section of the atrium wall; note that arterial smooth muscle fibers were not stained (green arrow). (B) Longitudinal section of the myocardium. C. Section showing myocardium and endocardium (red arrow). DAB product is brown and the counterstain is cresyl violet.Application
WB analysis of Kv11.1 transfected HEK293 lysates using Kv11.1 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.| Product Name | Kv11.1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Kv11.1This gene encodes a voltage-activated potassium channel belonging to the eag family. It shares sequence similarity with the Drosophila ether-a-go-go (eag) gene. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide AFLLKETEEGPPATEC corresponding to residues 430-445 of human KV11.1 (HERG) (Accession Q12809 ). Extracellular, between S1 and S2 domains. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHCIPWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SQT1 KCNH2 potassium voltage-gated channel subfamily H (eag-related) member 2 HERG1 LQT2 HERG ERG1 |
|---|---|
| Target | Kv11.1 |
Application
FACS analysis of K562 cells using Kv11.1 antibody (black) or an isotype control (green).
Application
ICC/IF analysis of Kv11.1 transfected HEK293 cells using Kv11.1 antibody at a dilution of 1:25 (red) in the absence (left) or presence (right) of blocking peptide. Nuclei were stained with Hoechst 33342 (blue).
Application
IP analysis was performed with Kv11.1 expressing HEK293 lysates and Kv11.1 antibodies (lane 2), (lane 3), (lane 4) or pre-immune serum (lane 5). Control lysate (lane 1) and the precipitates were detected by . Red arrow indicates Kv11.1 while the black arrow shows the IgG heavy chain.
Application
IHC analysis of rat heart using Kv11.1 antibody. (A) Transversal section of the atrium wall; note that arterial smooth muscle fibers were not stained (green arrow). (B) Longitudinal section of the myocardium. C. Section showing myocardium and endocardium (red arrow). DAB product is brown and the counterstain is cresyl violet.
Application
WB analysis of Kv11.1 transfected HEK293 lysates using Kv11.1 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
