| Product Name | l + S Afadin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to l + S AfadinAfadin is a cell-cell adherens junction F-actin binding multidomain protein. Two splicing variants of afadin have been described: l-afadin, which is ubiquitously expressed, and the smaller s-afadin, which is abundantly expressed in neural tissue. l Afadin is a protein containing one PDZ domain in its middle part followed by three proline-rich and one F-actin-binding region at the C-terminal. s Afadin is a protein which lacks both the third proline-rich and the F-actin binding regions and is homologous to the human AF6 (ALL1 fusion partner from chromosome 6) gene product. |
| Immunogen | Synthetic peptide: CKLPGDDRLMKNRADHRS conjugated to maleimide-activated BSA, corresponding to amino acids 1163-1179 of Rat l + S Afadin (with N-terminal added cysteine). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| IF: Use at a dilution of 1/500 - 1/250. WB: Use at a dilution of 1/1000. Detects a band of approximately 200 (l) or 190 (s) kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Af6 |
|---|---|
| Target | l + S Afadin |
| Tissue Specificity | Anti l/s Afadin specifically recognizes l afadin and s afadin by immunoblotting (approximately 200 and 190 kDa respectively). Staining of the l/s afadin bands is specifically inhibited by the immunizing peptide. |
| Application Notes | IF: Use at a dilution of 1/500 - 1/250. WB: Use at a dilution of 1/1000. Detects a band of approximately 200 (l) or 190 (s) kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
