| Product Name | l Afadin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to l AfadinAfadin is a cell-cell adherens junction F-actin binding multidomain protein. Two splice variants of afadin have been described: l-afadin, which is ubiquitously expressed, and the smaller s-afadin, which is abundantly expressed in neural tissue. l-Afadin contains one PDZ domain near its middle followed by three proline-rich and one F-actin-binding region at the carboxyl-terminal. l/s-Afadin is localized to cell-cell junctions and binds several cytoplasmic proteins such as the small GTPase Ras, the tight junction protein ZO-1, and the vinculin binding protein ponsin/SH3P12. |
| Immunogen | Synthetic peptide conjugated to maleimide-activated KLH, corresponding to amino acids 1814-1829 of Rat I Afadin with N-terminal cysteine. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| IF: Use at a dilution of 1/500 - 1/1000. IHC: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 200 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Af6 |
|---|---|
| Target | l Afadin |
| Application Notes | IF: Use at a dilution of 1/500 - 1/1000. IHC: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 200 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
