Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.Application
HEK293T cells transfected with either L1CAM overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody , and then analyzed by flow cytometry.Application
Anti-L1CAM mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with L1CAMApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.| Product Name | L1CAM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1H3] to L1CAMCell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. |
| Immunogen | Protein expressed in 293T cell transfected with human L1CAM expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1H3 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| IHC 1:50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | L1CAM SPG1 NCAML1 HSAS MASA CAML1 S10 CD171 HSAS1 MIC5 N-CAML1 N CAML1 |
|---|---|
| Target | L1CAM |
| Tissue Specificity | is specific for human L1CAM. |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
Application
HEK293T cells transfected with either L1CAM overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody , and then analyzed by flow cytometry.
Application
Anti-L1CAM mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with L1CAM
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.| Application Notes | IHC 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
