Application
Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Lamin A + C antibody detects Lamin A + C protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Lamin A + C antibody diluted at 1:250.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Lamin A + C antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.Application
Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using Lamin A + C antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Lamin A + C antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Lamin A + C antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments. IP samples: HeLa whole cell extract A. 50 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of Lamin A + C protein by 4 μg Lamin A + C antibody 7.5 % SDS-PAGE The immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | Lamin A + C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Lamin A + CThe nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Lamin A/C. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Rhesus Monkey, Chimpanzee, BovineXenopus laevis (81%), Dog (98%), Pig (98%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDCD1 antibody CDDC antibody CMD1A antibody CMT2B1 antibody EMD2 antibody FPL antibody FPLD antibody HGPS antibody IDC antibody LDP1 antibody LFP antibody LGMD1B antibody LMN1 antibody LMNC antibody LMNL1 antibody PRO1 antibody LMNA antibod |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Lamin A + C antibody detects Lamin A + C protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Lamin A + C antibody diluted at 1:250.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Lamin A + C antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using Lamin A + C antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Lamin A + C antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Lamin A + C antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments. IP samples: HeLa whole cell extract A. 50 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of Lamin A + C protein by 4 μg Lamin A + C antibody 7.5 % SDS-PAGE The immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Positive Control | A431 , NIH-3T3 , *K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
