| Product Name | Lamin A antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [X67, X167, X233] to Lamin ANuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A type lamins coincides with cell differentiation and as A type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A type lamins. |
| Immunogen | cell preparation: Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | X67, X167, X233 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FWB |
| IF: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 60-75 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRatXenopusBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | EMD2 HGPS LMN A FPLD lamin A/C LDP1 LMN 1 LMNA FPL LMNC LMN C LFP NY REN 32 antigen CDCD1 IDC LGMD1B CMT2B1 NY REN32 antigen Lamin A PRO1 70 kDa lamin CMD1A LMN1 CDDC |
|---|---|
| Target | Lamin A |
| Tissue Specificity | The monoclonal antibody cocktail recognizes the karyoskeleton, i.e. the intermediate filament proteins of the nucleus. Reacts with Lamin isotypes of Mr 60-75 kDa (LA, LI, LII, LIII, B1, B2, C) |
| Cellular Localization | Nuclear |
| Application Notes | IF: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 60-75 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C. |
| Storage Buffer | Preservative: 0.09% Sodium Azide. Constituents: Tissue culture supernatant |
