Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [GT144] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Lamin B2 antibody [GT144] detects LMNB2 protein at nuclear envelope by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min. Red: LMNB2 protein stained by Lamin B2 antibody [GT144] diluted at 1:1000. Green: alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]Application
Lamin B2 antibody [GT144] detects LMNB2 protein at nucleus membrane on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Lamin B2 antibody [GT144] dilution: 1:200.Application
Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract D. 30 µg SK-N-AS whole cell lysate/extract 7.5% SDS-PAGE Lamin B2 antibody [GT144] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract B. 30 µg HepG2 cytosol lysate/extract C. 30 µg HepG2 nuclear lysate/extract 7.5% SDS-PAGE Lamin B2 antibody [GT144] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Lamin B2 antibody [GT144] immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 μg of Lamin B2 antibody [GT144] 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [GT144] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Product Name | Lamin B2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to Lamin B2 (lamin B2)The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Lamin B2. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT144 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Molecular Weight(MW) | 68 kDa (note) |
|---|---|
| Cellular Localization | Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side |
Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [GT144] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Lamin B2 antibody [GT144] detects LMNB2 protein at nuclear envelope by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min. Red: LMNB2 protein stained by Lamin B2 antibody [GT144] diluted at 1:1000. Green: alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]
Application
Lamin B2 antibody [GT144] detects LMNB2 protein at nucleus membrane on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Lamin B2 antibody [GT144] dilution: 1:200.
Application
Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract D. 30 µg SK-N-AS whole cell lysate/extract 7.5% SDS-PAGE Lamin B2 antibody [GT144] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract B. 30 µg HepG2 cytosol lysate/extract C. 30 µg HepG2 nuclear lysate/extract 7.5% SDS-PAGE Lamin B2 antibody [GT144] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Lamin B2 antibody [GT144] immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 μg of Lamin B2 antibody [GT144] 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [GT144] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS , HepG2 , HepG2 nuclear |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
