Application
Formalin-fixed, paraffin-embedded human tongue section, enzyme-treated was stained with 1:2,000 Monoclonal Anti-Laminin Clone: LAM-89 . The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.Application
Formalin-fixed, paraffin-embedded human placenta section, enzyme-treated was stained with 1:2,000 Monoclonal Anti-Laminin Clone: LAM-89 . The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.| Product Name | Laminin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [LAM - 89] to LamininLaminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with an approximate molecular weight of 900 kD. It plays an important role in many aspects of the cell biology. Laminin is composed of one A chain (400 kD) one B1 chain (215 kD) and one B2 chain (205 kD) all held together by disulfide bonds. The molecule has a cross-like form with globular units near the ends of each chain, the sites where it is bound to Collagen IV, heparan sulfate, proteoglycan as well as to the surface of epithelial cells. Laminins from various species have common antigenic determinants. Laminin is only found in significant quantities in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. It has been found to modulate cell differentiation, cell shaping and also cell movement because it appears to be an important cell substrate-adhesion protein. Variations in the expression of this protein have been observed in embryogenesis, organogenesis, post traumatic healing and cancer. The greatest interest in laminin has been provoked by the discovery of its ability to promote neurite regeneration. |
| Immunogen | Purified protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | LAM-89 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-P |
| IF: Use at a dilution of 1/1000 . Can be used for protease-digested formalin-fixed, paraffin embedded human or animal tissue sections. Localizes the basal membrane of blood vessels, epithelium, nerve, and muscle fibers. IHC-P: Use at a dilution of 1/1000. This concentration was determined by immunoperoxidase staining of protease-digested human tissue. Antigen unmasking is critical in using this product. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanPigCat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Laminin |
|---|---|
| Tissue Specificity | In a dot blot assay, the product shows no reaction with Collagen IV, Fibronectin, Vitronectin or Chondroitin sulfate types A, B, and C. |
| Cellular Localization | Extracellular. Found in the basement membranes. |
Application
Formalin-fixed, paraffin-embedded human tongue section, enzyme-treated was stained with 1:2,000 Monoclonal Anti-Laminin Clone: LAM-89 . The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Application
Formalin-fixed, paraffin-embedded human placenta section, enzyme-treated was stained with 1:2,000 Monoclonal Anti-Laminin Clone: LAM-89 . The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.| Application Notes | IF: Use at a dilution of 1/1000 . Can be used for protease-digested formalin-fixed, paraffin embedded human or animal tissue sections. Localizes the basal membrane of blood vessels, epithelium, nerve, and muscle fibers. IHC-P: Use at a dilution of 1/1000. This concentration was determined by immunoperoxidase staining of protease-digested human tissue. Antigen unmasking is critical in using this product. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Ascites fluid. |
