| Product Name | LAMP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [LY1C6] to LAMP1 - Lysosome MarkerLysosome associated membrane proteins (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMC to the site of inflammation. |
| Immunogen | This antibody was derived from mice that were immunized with rat liver lysosome membranes as the source of the full length LAMP1 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | LY1C6 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| ICC: Use at a dilution of 1/100 - 1/750. IP:Use at 5ug/ml. WB: Use at a concentration of 1 μg/ml. IHC:Use at an dependent assay.Detects a band of approximately 120 kDa.Cross react to human only validated by ICC/IF. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatHamster |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | LGP120 |
|---|---|
| Target | LAMP1 |
| Cellular Localization | Type I membrane protein. Lysosomal. This protein shuttles between lysosomes, endosomes, and the plasma membrane. |
| Positive Control | Highly purified rat lysosomal membrane preparations |
|---|---|
| Application Notes | ICC: Use at a dilution of 1/100 - 1/750. IP:Use at 5ug/ml. WB: Use at a concentration of 1 μg/ml. IHC:Use at an dependent assay.Detects a band of approximately 120 kDa.Cross react to human only validated by ICC/IF. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2 |
