Application
Immunofluorescent analysis of LAP1 using anti-LAP1 monoclonal antibody shows staining in NS-1 Cells.Application
Immunohistochemistry was performed on rat breast tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:50 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.Application
Immunohistochemistry was performed on rat colon tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.Application
Immunohistochemistry was performed on rat lymph node tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:50 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.| Product Name | LAP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to LAP1In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B & 1C share a high degree of sequence homology while LAP 2 is distinct from the others. LAP 1A & B bind lamins A, C and B1, while LAP 2 binds lamin B1 only. In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly. |
| Immunogen | Pore complex-lamina fraction isolated from rat liver nuclear envelopes. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | RL13 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| IHC-P: 1:20 - 1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | Anpep AP-M AP-N Apm Apn KZP Lap1 rAPN alanyl (membrane) aminopeptidase |
|---|---|
| Target | LAP1 |
Application
Immunofluorescent analysis of LAP1 using anti-LAP1 monoclonal antibody shows staining in NS-1 Cells.
Application
Immunohistochemistry was performed on rat breast tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:50 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.
Application
Immunohistochemistry was performed on rat colon tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.
Application
Immunohistochemistry was performed on rat lymph node tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:50 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.| Application Notes | IHC-P: 1:20 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | (13.8 mg/ml) in PBS containing 0.05% sodium azide. |
