Application
Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets. LAT and NTAL were detected on RBL rat basophilic leukemia cell line (1B) by anti-LAT (LAT-1D1) and polyclonal anti-NTAL antibody , followed by 10 nm gold-conjugated secondary antibodies.Application
Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets. LAT and NTAL were detected on mouse bone marrow-derived mast cells (BMMC; 1A) and RBL rat basophilic leukemia cell line (1B) by anti-LAT (LAT-1D1) and polyclonal anti-NTAL antibody, followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively.| Product Name | LAT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [LAT-1D1] to LATLAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation. Entrez Gene (mouse): 7 F3Entrez Gene (rat): 1q36 |
| Immunogen | Bacterially expressed full-length rat LAT |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | LAT-1D1 |
| Host Species | Mouse |
| Tested Applications | EMICC/IFIPWB |
| WB: Use at 5-10 µg/mL. Positive Control: mouse spleen: |
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| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Target | LAT |
|---|---|
| Tissue Specificity | The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), a transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. |
| Cellular Localization | Present in lipid rafts. |
Application
Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets. LAT and NTAL were detected on RBL rat basophilic leukemia cell line (1B) by anti-LAT (LAT-1D1) and polyclonal anti-NTAL antibody , followed by 10 nm gold-conjugated secondary antibodies.
Application
Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets. LAT and NTAL were detected on mouse bone marrow-derived mast cells (BMMC; 1A) and RBL rat basophilic leukemia cell line (1B) by anti-LAT (LAT-1D1) and polyclonal anti-NTAL antibody, followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively.| Application Notes | WB: Use at 5-10 µg/mL. Positive Control: mouse spleen: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
