Application
Immunofluorescence analysis of HepG2 cells, using LATS2 Antibody. The picture on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using LATS2 Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | LATS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LATS2Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human LATS2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-P |
| IF: 1:100~1:500 : |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LATS2 EC2.7.11.1 Serine/threonineprotein kinase kpm Wartslike kinase Large tumor suppressor homolog 2 Warts-like kinase Large tumor suppressor homolog2 Serine/threonine-protein kinase kpm EC 2.7.11.1 Kinase phosphorylated during mitosis protein W |
|---|---|
| Target | LATS2 |
| Molecular Weight(MW) | 120 kDa (note) |
| Tissue Specificity | LATS2 Antibody detects endogenous levels of total LATS2 protein. |
| Cellular Localization | Co-localizes with STK6 at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. |
Application
Immunofluorescence analysis of HepG2 cells, using LATS2 Antibody. The picture on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using LATS2 Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IF: 1:100~1:500 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
