Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with LATS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Antibody is used in Western blot to detect LATS2 in NIH-3T3 cell lysate.| Product Name | LATS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Warts-like kinase, LATS2 {ECO:0000312|EMBL:BAA923811}, KPM |
| Product description | LATS2 is a serine/threonine protein kinase belonging to the LATS tumor suppressor family. This protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression.1) Blume-Jensen P, et al. Nature 2001. 411: 355. Cantrell D, J. Cell Sci. 2001. 114: 1439. JhiangOncogene 2000. 19: 5590. Manning G, et al. Science 2002. 298: 1912. Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359. Robertson, S. et al. Trends Genet. 2000. 16: 368. Robinson D, et al. Oncogene 2000. 19: 5548. Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889. Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561. Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271. |
| Immunogen | This LATS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-258 amino acids from the Central region of human LATS2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | LATS2 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase LATS2 Kinase phosphorylated during mitosis protein Large tumor suppressor homolog 2 Serine/threonine-protein kinase kpm Warts-like kinase LATS2 {ECO:0000312|EMBL:BAA923811} KPM |
| Molecular Weight(MW) | 120 kDa |
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with LATS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Antibody is used in Western blot to detect LATS2 in NIH-3T3 cell lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
