Application
Immunohistochemistry: Immunohistochemical staining of LC3 in mouse liver.Application
Western Blot: LC3 Antibody [ - Western Blot on human brain lysate.Application
Flow Cytometry: FACS staining of NTERA-2 cells using at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.| Product Name | LC3A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LC3ALC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might m |
| Immunogen | Genomic sequence made to a N-terminal portion of the human LC3 protein [Swiss-Prot# Q9H492] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHCWB |
| Western Blot 1:2500, Immunohistochemistry-Paraffin 1:100: |
|
| Species Reactivity | HumanMouseRatZebra FishBovine |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATG8E MAP1ALC3 LC3A LC3 MAP1BLC3 |
|---|---|
| Target | LC3 |
| Tissue Specificity | LC3 |
| Cellular Localization | Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle ? autophagosome membrane; Lipid-anchor. Note= LC3-II binds to the autophagic membranes. |
Application
Immunohistochemistry: Immunohistochemical staining of LC3 in mouse liver.
Application
Western Blot: LC3 Antibody [ - Western Blot on human brain lysate.
Application
Flow Cytometry: FACS staining of NTERA-2 cells using at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.| Application Notes | Western Blot 1:2500, Immunohistochemistry-Paraffin 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, 0.1% BSA and 50% glycerol |
