Application
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B protein stained by LC3B antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
LC3B antibody detects MAP1LC3B protein by western blot analysis. A. 20 µg Huh7 whole cell lysate/extract (untreated) B. 20 µg Huh7 whole cell lysate/extract (3uM-Thapsigargin treatment for 12hr) LC3B antibody dilution: 1:1500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
LC3B antibody detects LC3B protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. LC3B antibody diluted at 1:100.Application
LC3B antibody detects LC3B protein in HCV-infected samples by western blot analysis. A. 20 µg Huh7 whole cell lysate/extract (un-infected) B. 20 µg Huh7 whole cell lysate/extract (HCV-infected) LC3B antibody dilution: 1:1500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min. Green: LC3B protein stained by LC3B antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
LC3B antibody detects LC3B protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min. Brown: Unlabelled sample was also used as a control. Blue: LC3B antibody dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.Application
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B protein stained by LC3B antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
LC3B antibody detects MAP1LC3B protein by western blot analysis. A. 50 µg mouse brain lysate/extract 15% SDS-PAGE LC3B antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
LC3B antibody detects LC3B protein in Thapsigargin-treated samples by western blot analysis. Upper panel: LC3B antibody Lower panel: Beta-actin antibody A. 30 µg HepG2 whole cell lysate/extract (untreated) B. 30 µg HepG2 whole cell lysate/extract (3 µM Thapsigargin treatment for 12 hr) 15% SDS-PAGE LC3B antibody dilution: 1:1000 Beta-actin antibody dilution: 1:20000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HepG2 cells were untreated or treated with 3 μM thapsigargin for 16 and 24 hrs. Whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | LC3B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LC3B (microtubule-associated protein 1 light chain 3 beta)The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PIPWB |
| WB:1:250~1:1000 IP:1:50~1:250 ICC/IF:1:50~1:250 IHC-p:1:25~1:100 |
|
| Species Reactivity | HumanMousePigRat |
| Predicted Reactivity | Zebrafish, Japanese medaka, Rhesus Monkey, Chimpanzee, BovineZebrafish (100%), Japanese medaka (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATG8F antibody LC3B antibody MAP1A/1BLC3 antibody MAP1LC3B antibody autophagy-related ubiquitin-like modifier LC3 B antibody MAP1A/MAP1B LC3 B antibody MAP1A/MAP1B light chain 3 B antibody MAP1 light chain 3-like protein 2 antibody microtubule-asso |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Tissue Specificity | Cross-reactivity may occur with other LC3 isoforms |
| Cellular Localization | Cytoplasm , Endomembrane system; Lipid-anchor , Cytoplasmic vesicle , autophagosome membrane; Lipid-anchor |
Application
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B protein stained by LC3B antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
LC3B antibody detects MAP1LC3B protein by western blot analysis. A. 20 µg Huh7 whole cell lysate/extract (untreated) B. 20 µg Huh7 whole cell lysate/extract (3uM-Thapsigargin treatment for 12hr) LC3B antibody dilution: 1:1500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
LC3B antibody detects LC3B protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. LC3B antibody diluted at 1:100.
Application
LC3B antibody detects LC3B protein in HCV-infected samples by western blot analysis. A. 20 µg Huh7 whole cell lysate/extract (un-infected) B. 20 µg Huh7 whole cell lysate/extract (HCV-infected) LC3B antibody dilution: 1:1500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min. Green: LC3B protein stained by LC3B antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
LC3B antibody detects LC3B protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min. Brown: Unlabelled sample was also used as a control. Blue: LC3B antibody dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
Application
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B protein stained by LC3B antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
LC3B antibody detects MAP1LC3B protein by western blot analysis. A. 50 µg mouse brain lysate/extract 15% SDS-PAGE LC3B antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
LC3B antibody detects LC3B protein in Thapsigargin-treated samples by western blot analysis. Upper panel: LC3B antibody Lower panel: Beta-actin antibody A. 30 µg HepG2 whole cell lysate/extract (untreated) B. 30 µg HepG2 whole cell lysate/extract (3 µM Thapsigargin treatment for 12 hr) 15% SDS-PAGE LC3B antibody dilution: 1:1000 Beta-actin antibody dilution: 1:20000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HepG2 cells were untreated or treated with 3 μM thapsigargin for 16 and 24 hrs. Whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | NT2D1 , PC-3 , U87-MG , SK-N-SH , mouse brain , Rat brain , HepG2 (untreated) , HepG2 (3 μM Thapsigargin treatment for 12 hr) , HepG2 (3 μM Thapsigargin treatment for 16hr) , HepG2 (3 μM Thapsigargin treatment for 24hr) , Huh7 (un-infected) , Huh7 (HCV-infected) , HeLa , HeLa (50μM Chloroquine treatment for 24 hr) |
|---|---|
| Application Notes | WB:1:250~1:1000 IP:1:50~1:250 ICC/IF:1:50~1:250 IHC-p:1:25~1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
