Application
LC3B antibody [N1C3] detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min. Green: LC3B protein stained by LC3B antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
LC3B antibody [N1C3] detects LC3B protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min. Brown: Unlabelled sample was also used as a control. Blue: LC3B antibody [N1C3] dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.Application
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: Rat brain 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human Breast carcinoma, using LC3B antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: U87-MG 15% SDS PAGE diluted at 1:1000| Product Name | LC3B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LC3B (microtubule-associated protein 1 light chain 3 beta)The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq] |
| Immunogen | Full length human LC3B Recombinant protein. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Japanese medaka, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineZebrafish (91%), Japanese medaka (89%), Xenopus laevis (92%), Pig (99%), Chicken (95%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATG8F antibody LC3B antibody MAP1A/1BLC3 antibody MAP1LC3B antibody autophagy-related ubiquitin-like modifier LC3 B antibody MAP1A/MAP1B LC3 B antibody MAP1A/MAP1B light chain 3 B antibody MAP1 light chain 3-like protein 2 antibody microtubule-asso |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Endomembrane system; Lipid-anchor , Cytoplasmic vesicle , autophagosome membrane; Lipid-anchor |
Application
LC3B antibody [N1C3] detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min. Green: LC3B protein stained by LC3B antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
LC3B antibody [N1C3] detects LC3B protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min. Brown: Unlabelled sample was also used as a control. Blue: LC3B antibody [N1C3] dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: Rat brain 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human Breast carcinoma, using LC3B antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: U87-MG 15% SDS PAGE diluted at 1:1000| Positive Control | U87-MG , Mouse brain , Rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
