Application
ICC: Human LDL in HepG2 cells fixed in methanol.| Product Name | LDL Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [15C8] to LDL ReceptorThe low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. |
| Immunogen | A trypic digest of low density lipoprotein receptor from bovine |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 15C8 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-FIPWB |
| For ELISA: Use at a concentration of 0.5-1 μg/ml. For FACS: Use 5μg (undiluted) to label 10^6 clells - Incubate for 30min at 4oC. For ICC: Use at a dilution of 1-5 μg/ml. For IHC-Fr: Use at a dilution of 1-5 μg/ml. For IP: Use at a dilution of 1-2 μg/ml. For WB: Use at a dilution of 1-5 μg/ml. Predicted molecular weight: 160 kDa. Include 2mM Ca+2 with the antibody and avoid EDTA. Product GTX16897 has been reported to bind best to the unreduced LDL-R. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanBovine |
| Concentration | 1mg/ml |
| Target | LDL Receptor |
|---|---|
| Cellular Localization | Cell Membrane and Cytoplasmic |
| Light Chain Type | kappa |
Application
ICC: Human LDL in HepG2 cells fixed in methanol.| Positive Control | Liver sections, HT1080 cells, HepG2 cells - Negative controls: HepG2 cells and normal skin |
|---|---|
| Application Notes | For ELISA: Use at a concentration of 0.5-1 μg/ml. For FACS: Use 5μg (undiluted) to label 10^6 clells - Incubate for 30min at 4oC. For ICC: Use at a dilution of 1-5 μg/ml. For IHC-Fr: Use at a dilution of 1-5 μg/ml. For IP: Use at a dilution of 1-2 μg/ml. For WB: Use at a dilution of 1-5 μg/ml. Predicted molecular weight: 160 kDa. Include 2mM Ca+2 with the antibody and avoid EDTA. Product GTX16897 has been reported to bind best to the unreduced LDL-R. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 0.05M sodium phosphate containing 0.2% gelatin and 0.1% sodium azide |
