| Product Name | LEF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1C3.1D10] to LEF1Lymphoid enhancer factor, LEF1, is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF1, TCF1, TCF3 and TCF4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. |
| Immunogen | Synthetic peptide: TGIPHPAIVT PQVKQEHPHT DSDLMHVKPQ HEQRKEQEPK RP, corresponding to amino acids 256-297 of Human LEF1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1C3.1D10 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a concentration of 1 - 5 μg/ml. Predicted MW 55-57 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | DKFZp586H0919 FLJ46390 TCF1ALPHA |
|---|---|
| Target | LEF1 |
| Tissue Specificity | Recognizes all human LEF1 isoforms using Western blotting. The antibody does not cross-react with TCF1 or TCF4. |
| Cellular Localization | Nuclear |
| Application Notes | WB: Use at a concentration of 1 - 5 μg/ml. Predicted MW 55-57 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.01M PBS. pH 7.5 containing 0.08% Sodium Azide |
