| Product Name | LEF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [REMB1] to LEF1Lymphoid enhancer factor, LEF1, is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF1, TCF1, TCF3 and TCF4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. |
| Immunogen | Recombinant full length LEF1 (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | REMB1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| IF: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 5 μg/ml, this is recommended using whole extracts of human Jurkat cells or transiently expressed human LEF1 in whole cell lysates from Cos-1 cells. Predicted MW 55-57 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | DKFZp586H0919 FLJ46390 TCF1ALPHA |
|---|---|
| Target | LEF1 |
| Tissue Specificity | Recognizes the beta-Catenin Binding Domain of human LEF1 using immunoblotting (55-57 kDa). The antibody does not cross-react with TCF1 or TCF4. |
| Cellular Localization | Nuclear |
| Positive Control | Human Jurkat cells or transiently expressed human LEF1 in whole cell lysates from Cos-1 cells |
|---|---|
| Application Notes | IF: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 5 μg/ml, this is recommended using whole extracts of human Jurkat cells or transiently expressed human LEF1 in whole cell lysates from Cos-1 cells. Predicted MW 55-57 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 0.01M PBS. pH 7.5 containing 0.08% Sodium Azide |
