| Product Name | Leptin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4F8] to LeptinLeptin (from the Greek leptos, meaning thin) is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. |
| Immunogen | Recombinant full length protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4F8 |
| Host Species | Mouse |
| Tested Applications | ELISA |
| For ELISA: Use at an assay dependent dilution. This antibody was tested in sandwich ELISA as capture and detection antibodies with human blood samples. This antibody detects antigen in human blood with high sensitivity. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Target | Leptin |
|---|---|
| Tissue Specificity | This antibody recognizes recombinant human leptin and leptin circulating in human blood. |
| Cellular Localization | Secreted |
| Application Notes | For ELISA: Use at an assay dependent dilution. This antibody was tested in sandwich ELISA as capture and detection antibodies with human blood samples. This antibody detects antigen in human blood with high sensitivity. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide |
