Application
LIM homeobox protein 8 antibody immunoprecipitates LIM homeobox protein 8 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of LIM homeobox protein 8 protein by 2 μg of LIM homeobox protein 8 antibody 10% SDS-PAGE The immunoprecipitated LIM homeobox protein 8 protein was detected by LIM homeobox protein 8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE diluted at 1:5000| Product Name | LHX8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LHX8 (LIM homeobox 8)Members of the LIM homeobox gene family, such as LHX8, encode transcription regulators that share common structural features. They all contain 2 tandemly repeated cysteine-rich double-zinc finger motifs, called LIM domains, in addition to a homeodomain. The homeodomain is a DNA-binding domain, and the LIM domains are essential for regulating the activity of these molecules by interacting with other proteins. Members of the LIM homeobox gene family are required for the patterning or the specification and differentiation of different cell types during embryonic development (Zhao et al., 1999 [PubMed 10611327]).[supplied by OMIM] |
| Immunogen | Full length human LHX8 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, ChickenMouse (89%), Rat (88%), Pig (82%), Chicken (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Lhx7 antibody LHX8 antibody LIM-homeodomain protein Lhx8 antibody LIM/homeobox protein Lhx8 antibody LIM homeobox 8 antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) |
| Cellular Localization | Nucleus |
Application
LIM homeobox protein 8 antibody immunoprecipitates LIM homeobox protein 8 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of LIM homeobox protein 8 protein by 2 μg of LIM homeobox protein 8 antibody 10% SDS-PAGE The immunoprecipitated LIM homeobox protein 8 protein was detected by LIM homeobox protein 8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE diluted at 1:5000| Positive Control | Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
