Application
Immunohistochemistry staining of human spleen (paraffin sections) with anti-LIME polyclonal antibody .| Product Name | LIME antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to LIMELIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. |
| Immunogen | Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: Use at an assay dependent dilution. Predicted molecular weight: 32.3 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 54923 dJ583P15.4 LIME Q9H400 LIME1 FLJ20406 609809 LP8067 |
|---|---|
| Target | LIME |
Application
Immunohistochemistry staining of human spleen (paraffin sections) with anti-LIME polyclonal antibody .| Application Notes | WB: Use at an assay dependent dilution. Predicted molecular weight: 32.3 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
