Application
Western Blot analysis (reducing conditions) of human LIME using anti-human LIME . Lane 1: J77 cell line transfected with LIME Lane 2: non-transfected J77 cell line (cells are essentially devoid of endogenous LIME expression)| Product Name | LIME antibody [LIME-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [LIME - 10] to LIMELIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. |
| Immunogen | COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | LIME-10 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at an assay dependent dilution. Predicted molecular weight: 32.3 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | 54923, dJ583P15.4, LIME, Q9H400, LIME1, FLJ20406, 609809, LP8067 |
|---|---|
| Target | LIME |
Application
Western Blot analysis (reducing conditions) of human LIME using anti-human LIME . Lane 1: J77 cell line transfected with LIME Lane 2: non-transfected J77 cell line (cells are essentially devoid of endogenous LIME expression)| Application Notes | WB: Use at an assay dependent dilution. Predicted molecular weight: 32.3 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
