| Product Name | LIME antibody [mLIME-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [mLIME-05] to LIMELIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.Entrez Gene: 2 H4 |
| Immunogen | Recombinant protein corresponding to amino acids 86-202 of mouse LIME |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | mLIME-05 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: Positive Control: murine splenic T cells | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Target | LIME |
|---|---|
| Tissue Specificity | Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells. |
| Cellular Localization | Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T-cell with antigen-presenting cell. |
| Application Notes | IP: Positive Control: murine splenic T cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
