Application
Immunocytochemistry/Immunofluorescence: SR-BII Antibody - SR-BII antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).Application
'Western Blot: SR-BII Antibody - Western blot analysis of SR-BII in 1. Human heart lysate 2. Mouse heart lysate'| Product Name | LIMP II antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to LIMP IIHigh density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain is almost completely different. SR-BII binds HDLs and mediates selective uptake of HDL cholesteryl ester, but with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressing SR-BI, with SR-BII expression found in liver, adrenal glands, and testes. It is located primarily in limiting membranes of lysosomes and endosomes. |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 450-478 of Mouse SR-BII (UniProt ID# O35114). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| IF: Use at a dilution of 1/500 - 1/1000. IHC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Predicted molecular weight: 82 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | MouseRatHamsterBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Scarb2 Cd36l2 LIMP2 12492 SRBII LIMP-2 MLGP85 LGP85 9330185J12Rik LIMP 2 |
|---|---|
| Target | SR-BII |
| Tissue Specificity | is specific for the SR-BII. |
| Cellular Localization | Lysosomal membrane |
Application
Immunocytochemistry/Immunofluorescence: SR-BII Antibody - SR-BII antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Application
'Western Blot: SR-BII Antibody - Western blot analysis of SR-BII in 1. Human heart lysate 2. Mouse heart lysate'| Application Notes | IF: Use at a dilution of 1/500 - 1/1000. IHC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Predicted molecular weight: 82 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: Sodium Azide; Constituents: Whole Serum |
