Application
Immunocytochemistry/Immunofluorescence: LIMPII/lpg85 Antibody [ - Detection of LIMPII/lpg85 (Green) in Hela cells using Nuclei (Blue) are counterstained using Hoechst 33258.Application
Immunohistochemistry: LIMPII/lpg85 Antibody [ - Staining of LIMP II in rat infarctzone tissue with a DAB detection system.Application
Western Blot: LIMPII/lpg85 Antibody [ - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 (5), HEPG2 human liver lysate (6).| Product Name | LIMP II antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LIMP IILysosomal integral membrane protein II (LIMPII, also known as lpg85 and CD36L2) is a member of the scavenger receptor class B, member 2 family. LIMP II is ubiquitously expressed in most higher organisms and is a major lysosomal and endosomal membrane marker. The major portion of LIMPII resides on the luminal side and contains numerous N-glycosylation sites. To date, studies have suggested that this 478-residue type III glycoprotein may play a role as a scavenger receptor, in cell adhesion and fatty acid transportation similar to that of CD36. |
| Immunogen | A C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| For ICC: Use at a diltution of 1:250. For IHC-P (DAB): Use at a dilution of 1:200. For IP: Use at a dilution of 1:100. For WB (ECL): Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Scarb2 Cd36l2 LIMP2 12492 LIMPII/lgp85 LIMP-2 MLGP85 LGP85 9330185J12Rik LIMP 2 |
|---|---|
| Target | LIMPII/lgp85 |
| Tissue Specificity | This antibody is specific for mouse and human LIMPII/lgp85. |
Application
Immunocytochemistry/Immunofluorescence: LIMPII/lpg85 Antibody [ - Detection of LIMPII/lpg85 (Green) in Hela cells using Nuclei (Blue) are counterstained using Hoechst 33258.
Application
Immunohistochemistry: LIMPII/lpg85 Antibody [ - Staining of LIMP II in rat infarctzone tissue with a DAB detection system.
Application
Western Blot: LIMPII/lpg85 Antibody [ - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 (5), HEPG2 human liver lysate (6).| Application Notes | For ICC: Use at a diltution of 1:250. For IHC-P (DAB): Use at a dilution of 1:200. For IP: Use at a dilution of 1:100. For WB (ECL): Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Contains 0.05% Sodium Azide |
