Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LIMS1 antibody [C2C3], C-term diluted at 1:2000.| Product Name | LIMS1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LIMS1 (LIM and senescent cell antigen-like domains 1)The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human LIMS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | MouseRat |
| Predicted Reactivity | Human, Zebrafish, Xenopus laevis, Pig, ChickenHuman (100%), Zebrafish (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PINCH antibody PINCH1 antibody LIMS1 antibody renal carcinoma antigen NY-REN-48 antibody particularly interesting new Cys-His protein 1 antibody LIM and senescent cell antigen-like-containing domain protein 1 antibody LIM and senescent cell antigen-l |
|---|---|
| Target | Pinch-1 |
| Molecular Weight(MW) | 37 kDa (note) (6) |
| Cellular Localization | Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side |
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LIMS1 antibody [C2C3], C-term diluted at 1:2000.| Positive Control | Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
