Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Pinch 1 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000| Product Name | LIMS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Pinch 1 (LIM and senescent cell antigen-like domains 1)The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LIMS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chicken, BovineMouse (99%), Rat (99%), Zebrafish (95%), Xenopus laevis (97%), Pig (98%), Chicken (95%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PINCH antibody PINCH1 antibody LIMS1 antibody renal carcinoma antigen NY-REN-48 antibody particularly interesting new Cys-His protein 1 antibody LIM and senescent cell antigen-like-containing domain protein 1 antibody LIM and senescent cell antigen-l |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (7) |
Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Pinch 1 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
