Application
Western blot analysis using anti-LIN28 mAb against NTERA-2 cell lysate (1).Application
Confocal immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.Application
Confocal immunofluorescence analysis of NTERA-2 cells using anti-LIN28 mAb (green). Blue: DRAQ5 fluorescent DNA dye.| Product Name | Lin28A antibody [6D1F9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [6D1F9] to Lin28A |
| Immunogen | The immunogen corresponding to a region within amino acids 93 and 209 of LIN28 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6D1F9 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | CSDD1 , ZCCHC1 , LIN-28 , LIN-28A , LIN28A |
|---|---|
| Target | Lin28A |
| Molecular Weight(MW) | 23 kDa (note) |
| Cellular Localization | Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules. |
Application
Western blot analysis using anti-LIN28 mAb against NTERA-2 cell lysate (1).
Application
Confocal immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.
Application
Confocal immunofluorescence analysis of NTERA-2 cells using anti-LIN28 mAb (green). Blue: DRAQ5 fluorescent DNA dye.| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Antibody are purified by protein G affinity chromatography. Liquid in 0.01M Phosphate buffer, pH 7.4 containing 0.03% sodium azide. |
