Application
Sample (30 ug of whole cell lysate) A: Non-transfected HeLa lysates B: LIN28 transfected HeLa lysates 12% SDS PAGE diluted at 1:1000Application
LIN28 antibody detects LIN28 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: LIN28 protein stained by LIN28 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Lin28A antibody [GT717] detects Lin28A protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Lin28A antibody [GT717] dilution: 1:50Application
Lin28A antibody [GT717] detects LIN28 protein by Western blot analysis. A. 30 μg human ESC whole cell lysate/extract 12 % SDS-PAGE Lin28A antibody [GT717] dilution: 1:1000Application
Lin28A antibody detects Lin28A protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Lin28A antibody dilution: 1:100.Application
Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE diluted at 1:1000Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Lin28A antibody [GT717] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.| Product Name | Lin28A antibody [GT717] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT717] to Lin28A (lin-28 homolog A (C. elegans))Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LIN28. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT717 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Cat, Pig, Chicken, Sheep, BovineRat (98%), Cat (96%), Pig (96%), Chicken (82%), Sheep (97%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CSDD1 antibody, FLJ12457 antibody, LIN-28 antibody, LIN28 antibody, ZCCHC1 antibody, LIN28A antibody, zinc finger CCHC domain-containing protein 1 antibody, protein lin-28 homolog A antibody, zinc finger, CCHC domain containing 1 antibody, RNA-binding prot |
|---|---|
| Molecular Weight(MW) | 23 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus , nucleolus |
Application
Sample (30 ug of whole cell lysate) A: Non-transfected HeLa lysates B: LIN28 transfected HeLa lysates 12% SDS PAGE diluted at 1:1000
Application
LIN28 antibody detects LIN28 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: LIN28 protein stained by LIN28 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Lin28A antibody [GT717] detects Lin28A protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Lin28A antibody [GT717] dilution: 1:50
Application
Lin28A antibody [GT717] detects LIN28 protein by Western blot analysis. A. 30 μg human ESC whole cell lysate/extract 12 % SDS-PAGE Lin28A antibody [GT717] dilution: 1:1000
Application
Lin28A antibody detects Lin28A protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Lin28A antibody dilution: 1:100.
Application
Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE diluted at 1:1000
Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Lin28A antibody [GT717] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.| Positive Control | NT2D1 , human ESC , LIN28 transfected HeLa lysates , mouse placenta |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
