Application
Immunoprecipitation of LIN7A protein from MDA-MB-231 whole cell extracts using 5 μg of LIN7A antibody [GT879] . Western blot analysis was performed using LIN7A antibody [GT879] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
LIN7A antibody [GT879] detects LIN7A protein at junction by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LIN7A protein stained by LIN7A antibody [GT879] diluted at 1:500. Blue: Hoechst 33342 staining.Application
LIN7A antibody [GT879] detects LIN7A protein by western blot analysis. A. 30 μg MCF-7 whole cell extract B. 30 μgMDA-MB-231 whole cell extract 12 % SDS-PAGE LIN7A antibody [GT879] dilution: 1:1000| Product Name | LIN7A antibody [GT879] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT879] to LIN7A (lin-7 homolog A (C. elegans))Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LIN7A. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT879 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, BovineMouse (100%), Rat (100%), Zebrafish (97%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | VELI1 , TIP-33 , LIN-7A , lin-7 homolog A (C. elegans) , LIN7 , MALS-1 |
|---|---|
| Target | LIN7A |
| Molecular Weight(MW) | 26 kDa (note) (2) |
| Cellular Localization | Cell membrane; Peripheral membrane protein , Basolateral cell membrane; Peripheral membrane protein , Cell junction (By similarity) , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density; Peripheral membrane protein (By similarity) |
Application
Immunoprecipitation of LIN7A protein from MDA-MB-231 whole cell extracts using 5 μg of LIN7A antibody [GT879] . Western blot analysis was performed using LIN7A antibody [GT879] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
LIN7A antibody [GT879] detects LIN7A protein at junction by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LIN7A protein stained by LIN7A antibody [GT879] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
LIN7A antibody [GT879] detects LIN7A protein by western blot analysis. A. 30 μg MCF-7 whole cell extract B. 30 μgMDA-MB-231 whole cell extract 12 % SDS-PAGE LIN7A antibody [GT879] dilution: 1:1000| Positive Control | MCF-7 , MDA-MB-231 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
