Application
Cell lysates of HeLa(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human LITAF (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | LITAF antibody [AT1F9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT1F9] to LITAFLipopolysaccharide-induced tumor necrosis factor-alpha factor(LITAF) mediates the expression of inflammatory cytokines such as TNF-alpha in Lipopolysaccharide-induced processes. LITAF binds to STAT6B, a member of the STAT6 family forming a complex on the TNF-alpha promoter that modulates TNF activity. High levels of expression of LITAF mRNA have been observed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. |
| Immunogen | Recombinant human LITAF (1-161aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AT1F9 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:2000 ~ 3000. Optimal working concentrations should be determined experimentally by the end user. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | lipopolysaccharideinduced TNF factor, lipopolysaccharide-induced TNF factor, SIMPLE, LITAF, FLJ38636, TP53I7, PIG7 |
|---|---|
| Target | LITAF |
| Cellular Localization | Associated with membranes of lysosomes. |
| Light Chain Type | kappa |
Application
Cell lysates of HeLa(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human LITAF (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:2000 ~ 3000. Optimal working concentrations should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
