Application
Flow cytometric analysis of Hela cells, using anti-BIRC7 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Flow cytometric analysis of Jurkat cells, using anti-BIRC7 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC7.Application
Anti-BIRC7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BIRC7Application
Immunofluorescent staining of HeLa cells using anti-BIRC7 mouse monoclonal antibody .| Product Name | Livin antibody [1B10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1B10] to LivinProtects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. |
| Immunogen | Full length human recombinant protein of human Livin (NP_071444) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1B10 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MLIAP, KIAP, RNF50, LIVIN, ML-IAP, BIRC7, ML IAP |
|---|---|
| Target | Livin |
| Tissue Specificity | is specific for human Livin (NP_071444). |
| Cellular Localization | Nuclear, and in a filamentous pattern throughout the cytoplasm. |
Application
Flow cytometric analysis of Hela cells, using anti-BIRC7 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Flow cytometric analysis of Jurkat cells, using anti-BIRC7 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC7.
Application
Anti-BIRC7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BIRC7
Application
Immunofluorescent staining of HeLa cells using anti-BIRC7 mouse monoclonal antibody .| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
