Application
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LOX monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).Application
Anti-LOX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with LOXApplication
Immunohistochemical staining of paraffin-embedded colon tissue using anti-RC213323 mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.Application
Flow cytometric analysis of Hela cells, using anti-LOX antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Flow cytometric analysis of Jurkat cells, using anti-LOX antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
HEK293T cells transfected with either pCMV6-ENTRY LOX| Product Name | LOX antibody [5C4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5C4] to LOXResponsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 22-168 of human LOX (NP_002308) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5C4 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| ICC/IF 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC105112 |
|---|---|
| Target | LOX |
| Tissue Specificity | is specific for amino acids 22-168 of human LOX (NP_002308). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LOX monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Application
Anti-LOX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with LOX
Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-RC213323 mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.
Application
Flow cytometric analysis of Hela cells, using anti-LOX antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Flow cytometric analysis of Jurkat cells, using anti-LOX antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
HEK293T cells transfected with either pCMV6-ENTRY LOX| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
