Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
LYN antibody detects LYN protein by Western blot analysis. A. 30 µg THP-1 whole cell lysate/extract 7.5 % SDS-PAGE LYN antibody dilution: 1:1000Application
LYN antibody immunoprecipitates LYN protein in IP experiments. IP samples: K562 whole cell extract A. 40 μg K562 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of LYN protein by 4 μg LYN antibody 5 % SDS-PAGE The immunoprecipitated LYN protein was detected by LYN antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | LYN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LYN (v-yes-1 Yamaguchi sarcoma viral related oncogene homolog) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Sheep, BovineMouse (95%), Rat (95%), Xenopus laevis (81%), Chicken (85%), Sheep (96%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ26625 antibody, JTK8 antibody, LYN antibody, lck/Yes-related novel protein tyrosine kinase antibody, tyrosine-protein kinase Lyn antibody, v-yes-1 Yamaguchi sarcoma viral related oncogene homolog antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (2) |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
LYN antibody detects LYN protein by Western blot analysis. A. 30 µg THP-1 whole cell lysate/extract 7.5 % SDS-PAGE LYN antibody dilution: 1:1000
Application
LYN antibody immunoprecipitates LYN protein in IP experiments. IP samples: K562 whole cell extract A. 40 μg K562 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of LYN protein by 4 μg LYN antibody 5 % SDS-PAGE The immunoprecipitated LYN protein was detected by LYN antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
