Application
Immunofluorescence analysis of methanol-fixed A431, using LYN antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000Application
LYN antibody [N2C3] detects LYN protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LYN antibody [N2C3] diluted at 1:500.Application
LYN antibody detects LYN protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody at a dilution of 1:1000.| Product Name | LYN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LYN (v-yes-1 Yamaguchi sarcoma viral related oncogene homolog) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chicken, Sheep, BovineRat (97%), Zebrafish (84%), Xenopus laevis (90%), Chicken (92%), Sheep (99%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ26625 antibody, JTK8 antibody, LYN antibody, lck/Yes-related novel protein tyrosine kinase antibody, tyrosine-protein kinase Lyn antibody, v-yes-1 Yamaguchi sarcoma viral related oncogene homolog antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (2) |
Application
Immunofluorescence analysis of methanol-fixed A431, using LYN antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000
Application
LYN antibody [N2C3] detects LYN protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LYN antibody [N2C3] diluted at 1:500.
Application
LYN antibody detects LYN protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody at a dilution of 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
