Application
Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kDa| Product Name | Lys 20p + 21p antibody [31F5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [31F5] to Lys 20p + 21pLys20p and Lys21p are isozymes of yeast homocitrate synthase. They are the products of two distinct genes producing proteins of molecular weight 47.1 kDa in the case of Lys20p and 48.6kDa for Lys21p. However Lys20p and Lys21p are ~90% identical at the amino acid level and MCA-31F5 detects both proteins, revealing a doublet of stained bands on blots. |
| Immunogen | This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting. Nucleolar prep contains Lys20p protein (as well as Nop1p/fibrillarin). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 31F5 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| IF: Use at a dilution of 1/1000 - 1/10000 (yeast cells). WB: Use at a dilution of 1/10000 - 1/50000 (yeast protein samples, ECL detection). Detects bands of approximately 47.1 kDa Lys 20p and 48.6 kDa Lys 21p. For non-ECL detection, use at a dilution of 1/1000 - 1/5000. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Yeast |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Lys 20p + 21p |
|---|---|
| Cellular Localization | Nuclear |
Application
Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kDa| Application Notes | IF: Use at a dilution of 1/1000 - 1/10000 (yeast cells). WB: Use at a dilution of 1/10000 - 1/50000 (yeast protein samples, ECL detection). Detects bands of approximately 47.1 kDa Lys 20p and 48.6 kDa Lys 21p. For non-ECL detection, use at a dilution of 1/1000 - 1/5000. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 10mM Sodium Azide; Constituents: Raw Ascites |
