Application
Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor 2 Antibody (31-1D1) [ - Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monoclonal Antibody recognizing Muscarinic Acetylcholine Receptor M2 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting| Product Name | mAChR M2 antibody [31-1D1] |
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| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [31-1D1] to mAChR M2Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The mAChRs are present in neurons of the central and peripheral nervous systems, cardiac and smooth muscle and various exocrine glands. There are 5 subtypes (m1-m5) of the receptor that have a tissue specific pattern of expression. The m2 receptor is localized primarily in cardiac tissue and is also expressed at low levels in the hippocampus, cortex, striatum, thalamus, basal forebrain, brainstem, lung, vas deferens and uterus. |
| Immunogen | Purified porcine cardiac m2 muscarinic acetylcholine receptor. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 31-1D1 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| We recommend the following starting dilutions: immunoprecipitation, Western Blot. Optimal concentration should be determined experimentally by the end user. | |
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC120006, Cholinergic receptor, CHRM2, CHRM 2, muscarinic 2, M, isoform a, 7TM receptor, Muscarinic M2 receptor, Cholinergic receptor muscarinic 2, FLJ43243, muscarinic2, MGC120007, Cholinergic receptor muscarinic2, HM 2, 1129, Muscarinic acetylcholine re |
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| Target | mAChR M2 |
| Tissue Specificity | This is specific for the m2 mAChR subtype. |
| Cellular Localization | Plasma membrane |
Application
Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor 2 Antibody (31-1D1) [ - Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monoclonal Antibody recognizing Muscarinic Acetylcholine Receptor M2 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting| Application Notes | We recommend the following starting dilutions: immunoprecipitation, Western Blot. Optimal concentration should be determined experimentally by the end user. |
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| Form | Liquid |
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| Storage Instructions | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS |
