| Product Name | Macrophage antibody [LN-5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [LN - 5] to MacrophagesMacrophages and histiocytes, which are widely distributed throughout the body, play a crucial role in the body defense mechanisms. They are heterogenous in morphology, cytochemistry, function and surface antigens. Immunohistochemical labeling with monoclonal antibodies is one of the most specific and reliable techniques for the identification and localization of these cells. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and in the diagnosis of various inflammatory, reactive and neoplastic disorders of macrophages. |
| Immunogen | Deparaffinized human peripheral blood mononuclear cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | LN-5 |
| Host Species | Mouse |
| Tested Applications | ICC/IF |
| IF: Use at a dilution of 1/400. The epitope recognized by the product is resistant to formalin fixation and paraffin embedding. The antibody also may be used on material fixed with B5 solution, neutral buffered formalin, Zinc-Formalin, Brunnel's, Susa and Bouin's solutions. The antigen is not detectable by the antibody in standard frozen sections. Using indirect immunofluorescent or immunoperoxidase methods, the antibody stains the cytoplasm of macrophages and histiocytes in hematopoietic organs and Kupffer cells of the liver and Langerhans cells of the skin. Mantle zone B lymphocytes, interdigitating reticulum cells of lymph nodes and B cells in spleen are stained. Bone marrow lymphoid and myeloid precursor cells stain with a uniform interstitial pattern. Certain normal non-lymphoid cells such as spermatogonia are stained while others such as breast ductal epithelium, kidney tubular epithelium, endometrium, parathyroid and stomach chief cells are only weakly stained. The antibody is reactive with benign and malignant histiocytic lesions such as sinus histiocytosis, dermatopathic lymphadenopathy, persistent generalized lymphadenopathy, tuberculosis, sarcoidosis, Gaucher's disease, true histiocytic lymphomas, malignant histiocytosis and histiocytosis-X. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Macrophage |
|---|---|
| Tissue Specificity | Monoclonal Anti-Human Macrophages recognizes an antigen present in the cytoplasm of human macrophages in a wide variety of tissues and organs. |
| Application Notes | IF: Use at a dilution of 1/400. The epitope recognized by the product is resistant to formalin fixation and paraffin embedding. The antibody also may be used on material fixed with B5 solution, neutral buffered formalin, Zinc-Formalin, Brunnel's, Susa and Bouin's solutions. The antigen is not detectable by the antibody in standard frozen sections. Using indirect immunofluorescent or immunoperoxidase methods, the antibody stains the cytoplasm of macrophages and histiocytes in hematopoietic organs and Kupffer cells of the liver and Langerhans cells of the skin. Mantle zone B lymphocytes, interdigitating reticulum cells of lymph nodes and B cells in spleen are stained. Bone marrow lymphoid and myeloid precursor cells stain with a uniform interstitial pattern. Certain normal non-lymphoid cells such as spermatogonia are stained while others such as breast ductal epithelium, kidney tubular epithelium, endometrium, parathyroid and stomach chief cells are only weakly stained. The antibody is reactive with benign and malignant histiocytic lesions such as sinus histiocytosis, dermatopathic lymphadenopathy, persistent generalized lymphadenopathy, tuberculosis, sarcoidosis, Gaucher's disease, true histiocytic lymphomas, malignant histiocytosis and histiocytosis-X. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide |
