| Product Name | Macrophage + Monocytes antibody [MAC387] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [MAC387] to Macrophage + MonocyteMouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al. 1987) subsequent studies indicate that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al. 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha. The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cellds in a wide range of species. |
| Immunogen | Human monocytes |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MAC387 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIHC-FIHC-P |
| For Flow Cytometry: Use at a dilution of 1:50 - 1:100. For IHC-Fr and IHC-P: Use at a dilution of 1:100 - 1:200. Optimal dilutions/concentrations should be determined by the researcher.** This product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment prior to staining of paraffin sections. | |
| Species Reactivity | DogPigRatBaboonCatGoatGuinea PigHorseMinkRabbitBovineRhesus MonkeyCynomolgus MonkeyMarmoset |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | L1 |
|---|---|
| Tissue Specificity | The antigen recognized by clone MCA387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. |
| Application Notes | For Flow Cytometry: Use at a dilution of 1:50 - 1:100. For IHC-Fr and IHC-P: Use at a dilution of 1:100 - 1:200. Optimal dilutions/concentrations should be determined by the researcher.** This product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment prior to staining of paraffin sections. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
